yellow fever virus yfv synthetic rna Search Results


yfv synthetic rna standard  (ATCC)
94
ATCC yfv synthetic rna standard
(A-D) Pre-infection plasma samples prior to DF (green; n=31) vs. DHF/DSS (gold; n=33) were incubated with mature DENV2, mature DENV3, ZIKV, and <t>YFV</t> virions. (A,B) Left panel, 1:10 dlution of plasma; right panel, 1:30 dilution of plasma. (E) Pre-inapparent (blue circles; n=30) and pre-symptomatic (orange circles; n=29) secondary DENV3 infection plasma samples were incubated with ZIKV virions. After incubation with plasma samples in the presence of guinea pig complement, mixtures were freeze-thawed once, followed by RNAse A digestion and <t>RNA</t> extraction from intact virions. Virion lysis was quantified by measuring viral RNA via RT-qPCR (to measure the number of genome copies) and calculated as percentage (%) of virion lysis as described in Methods. Shown are median NT 50 (middle line), 25 th to 75 th percentile (box), and 5 th to 95 th percentile (whiskers) as well as the raw data (points). Asterisks indicate Benjamini-Hochberg-adjusted p-values for Mann-Whitney U tests (****p < 0.0001, and ns, non-significant).
Yfv Synthetic Rna Standard, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A-D) Pre-infection plasma samples prior to DF (green; n=31) vs. DHF/DSS (gold; n=33) were incubated with mature DENV2, mature DENV3, ZIKV, and YFV virions. (A,B) Left panel, 1:10 dlution of plasma; right panel, 1:30 dilution of plasma. (E) Pre-inapparent (blue circles; n=30) and pre-symptomatic (orange circles; n=29) secondary DENV3 infection plasma samples were incubated with ZIKV virions. After incubation with plasma samples in the presence of guinea pig complement, mixtures were freeze-thawed once, followed by RNAse A digestion and RNA extraction from intact virions. Virion lysis was quantified by measuring viral RNA via RT-qPCR (to measure the number of genome copies) and calculated as percentage (%) of virion lysis as described in Methods. Shown are median NT 50 (middle line), 25 th to 75 th percentile (box), and 5 th to 95 th percentile (whiskers) as well as the raw data (points). Asterisks indicate Benjamini-Hochberg-adjusted p-values for Mann-Whitney U tests (****p < 0.0001, and ns, non-significant).

Journal: medRxiv

Article Title: Complement-dependent virion lysis mediated by dengue-Zika virus cross-reactive antibodies correlates with protection from severe dengue disease

doi: 10.1101/2024.06.03.24308395

Figure Lengend Snippet: (A-D) Pre-infection plasma samples prior to DF (green; n=31) vs. DHF/DSS (gold; n=33) were incubated with mature DENV2, mature DENV3, ZIKV, and YFV virions. (A,B) Left panel, 1:10 dlution of plasma; right panel, 1:30 dilution of plasma. (E) Pre-inapparent (blue circles; n=30) and pre-symptomatic (orange circles; n=29) secondary DENV3 infection plasma samples were incubated with ZIKV virions. After incubation with plasma samples in the presence of guinea pig complement, mixtures were freeze-thawed once, followed by RNAse A digestion and RNA extraction from intact virions. Virion lysis was quantified by measuring viral RNA via RT-qPCR (to measure the number of genome copies) and calculated as percentage (%) of virion lysis as described in Methods. Shown are median NT 50 (middle line), 25 th to 75 th percentile (box), and 5 th to 95 th percentile (whiskers) as well as the raw data (points). Asterisks indicate Benjamini-Hochberg-adjusted p-values for Mann-Whitney U tests (****p < 0.0001, and ns, non-significant).

Article Snippet: Standard curves were prepared using seven 10-fold dilutions of a DENV2 8891 synthetic fragment, DENV3 UNC3009 in-vitro transcribed RNA, ZIKV RNA standard (ATCC VR-3252SD) or YFV synthetic RNA standard (ATCC VR-3253SD).

Techniques: Infection, Clinical Proteomics, Incubation, RNA Extraction, Lysis, Quantitative RT-PCR, MANN-WHITNEY